There have been many researchers who have studied the relationship between palindromic sequences and protein structures. A = Palindrome, B = Loop, C = Stem Relationship between Sequence and Protein Structure When the complementary strand is read backwards, the sequence is 5’-GGATCC-3’ which is identical to the first one, making it a palindromic sequence.Īnother restriction enzyme called EcoR1 recognizes and cleaves the following palindromic sequence:ĥ’ – G A A T T C – 3’ 3’ – C T T A A G – 5’ Palindrome Image of a palindrome in a DNA structure. This is the sequence where the restriction endonuclease, BamHI, binds to and cleaves at a specific cleavage site. An example of a palindromic sequence is 5’-GGATCC-3’, which has a complementary strand, 3’-CCTAGG-5’. So if a sequence is palindromic, the nucleotide sequence of one strand would be the same as its reverse complementary strand. The pairing of nucleotides within the DNA double-helix is complementary which consist of Adenine (A) pairing with either Thymine (T) in DNA or Uracil (U) in RNA, while Cytosine (C) pairs with Guanine (G). It is also known as a palindrome or an inverted-reverse sequence. Palindromic Sequencing Ī palindromic sequence is a sequence made up of nucleic acids within double helix of DNA and/or RNA that is the same when read from 5’ to 3’ on one strand and 5’ to 3' on the other, complementary, strand. 1.4 Likelihood of palindromic sequences in proteins.1.3 Effect on genomic instability in yeast.1.2 Relationship between Sequence and Protein Structure. Here, for example, the single difference in the sequences can be eliminated (red for blue or vice versa). Repairs can then be made (probably by the mechanism of homologous recombination). All that is needed is to form a loop so that the two sequences line up side-by-side. This orientation and redundancy may help ensure that a deleterious mutation in one copy of the set can be repaired using the information in another copy of that set. GTGTT AAGGGTACCCAACACCCTC 5' - 3' GAGGGTGTTGGGTACCCT AAACAC. CACAA TTCCCATGGGTTGTGGGAG 3' - 5' CTCCCACAACCCATGGGA TTTGTG. (The dashes represent the thousands of base pairs that separate adjacent palindromes.)ĥ'. The human Y chromosome contains 7 sets of genes - each set containing from 2 to 6 nearly-identical genes - oriented back-to-back or head-to-head that is, they are inverted repeats like the portion shown here.
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